Diversity and communities of culturable endophytic fungi from the root holoparasite Balanophora polyandra Griff. and their antibacterial and antioxidant activities

Abstract Purpose Balanophora polyandra Griff. is a holoparasitic medicinal plant that produces compounds with antibacterial and antioxidant activities. Plant endophytic fungi are an abundant reservoir of bioactive metabolites for exploitation, increasing number novel being isolated from fungi. The present study investigated the diversity culturable roots holoparasite B. to explore active strains metabolites. In addition, activities 22 cultured were also evaluated. Methods identified according their colony morphology ITS-5.8S rDNA sequencing. TLC-MTT-Bioautography assays DPPH radical scavenging employed assess ethyl acetate extracts Results One hundred twenty-five , including 70 female samples 55 male samples. Of them, twenty-two distinct isolates representing 15 genera species based on ITS-rDNA genomic sequence successfully . genus Calonectria was most prevalent genus, CF% 18.3, followed by Clonostachys Botryosphaeria values 13.4 10.0, respectively. Interestingly, fungal exhibited broad-spectrum against gram-positive gram-negative bacteria, as well potential IC 50 ranging 0.45 6.90 mg/mL. Among endophytes Bpf-10 ( Diaporthe sp.) Bpf-11 showed strongest biological more secondary Conclusions This reported crude first time. results revealed contains diverse potentially produce natural great value agriculture pharmaceutical industries.